Category Archives: biology

This Week in PLOS NTDs and PLOS Pathogens: Climate, Chemicals and Vector Control, the Incubation of Buruli Ulcer, Supressing Viral Growth, and More By PLOS Neglected Tropical Diseases and PLOS Pathogens

Buruli ulcer’s mode of transmission is presently unknown, but in Victoria, Australia, endemic areas have been carefully mapped and patients are known to report single visits or defined exposures to these areas. From a retrospective review, Jason Trubiano and colleagues … Continue reading

Posted in biology | Tagged , | Leave a comment

New technique in RNA interference cuts a serious amounts of cost in genetic screens

There exists a new contender in neuro-scientific gene discovery, and yes it’s giving knockout mice a run their money. Researchers for the Rockefeller University have shown which a new technique using RNA interference has the capacity to find genes that … Continue reading

Posted in biology, science | Tagged , | Leave a comment

Measles’ Natural Nemesis: Cells Infected by Measles Virus Pull out a Heavy Weapon in the Form of the Enzyme

Scientists at The Scripps Research Institute have found that a known enzyme in cells protects against measles virus, likely by altering the virus’s genetic material, RNA. Cells lacking the enzyme become highly vulnerable to the virus’s destructive effects. The enzyme … Continue reading

Posted in biology | Tagged | Leave a comment

Optimized Manufacture of Lentivirus Using FuGENE® HD Transfection Reagent

Lentiviral vectors are ideally suited vehicles for just a wide range of research applications. Based on pseudotype, lentiviruses infect a variety of cell types, non-discriminately transducing both dividing and non-dividing cells. As opposed to other popular vector delivery systems, lentivirus … Continue reading

Posted in biology | Tagged , , | Leave a comment

German Researchers Find Sensitizer for Gemcitabine Using DECIPHER RNAi Libraries

Researchers for the German Cancer Research facility (DKFZ) used Cellecta’s shRNA DECIPHER libraries to spot genes that sensitize pancreatic cells to gemcitabine. Pancreatic cancer only has a 6% 5-year survival rate. Gemcitabine could be the standard treatment in partnership with … Continue reading

Posted in biology | Tagged , , | Leave a comment

Gene Cloning and Protein Expression

For your current PSI-2 target ORFs, boundaries defined by domain family analysis and some constructs are generated per target to optimize the means to get a successful outcome. Our permutation strategy will depend on extension with the N or C-termini … Continue reading

Posted in biology | Tagged , , , | Leave a comment

A Screen of shRNAs Targeting Tumor Suppressor Genes to name Factors Linked to Paclitaxel Sensitivity

RNA interference methodologies are already found in promising small to large scale screening projects. The technology has allowed researchers to execute gene-silencing experiments in time frames and target cells previously not possible. While siRNA screens became heard of, large scale … Continue reading

Posted in biology | Tagged , , , , | Leave a comment