Scientific study has discovered a method for simultaneously visualizing gene number and protein expression in individual cells. The fluorescence microscopy technique could permit a detailed analysis of the relationship between gene status and expression of the corresponding protein in cells and tissues, and bring a clearer knowledge of cancer as well as other complex diseases, in line with researchers who led case study.
The new way is referred to as fluorescent in situ gene protein assay. It combines traditional fluorescent in place hybridization (FISH) using the in place proximity ligation assay. That is effective at resolving individual protein molecules.
“To my knowledge, this can be the first technique which allows us to concurrently address gene activity and corresponding protein expression inside the same cells,” says co-PI Dr. Arnab Chakravarti, chair and professor of radiation oncology and co-director with the Brain Tumor Program on the Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute.
“To be able to resolve gene and protein-expression changes across a tumor could help us understand what drives tumor behavior overall,” Chakravarti says
The modern assay is described within the journal Neuro-Oncology. Just for this study, PI Dr. Markus Bredel, an associate professor with the University of Alabama Birmingham, and an adjunct associate professor radiation oncology on the OSUCCC – James, along with Chakravarti and their collaborators first assayed fixed human glioblastoma tumor cells, then paraffin-embedded human glioblastoma tissue. In the two cases, they assayed for over expression of any mutant sort of the epidermal growth factor receptor gene, EGFRvIII, and for amounts of its truncated protein in glioblastoma.
“Using this method has possibility to perform a detailed research into the relationship between cancer gene status and corresponding protein expression in cells and tissues,” Bredel says. “We demonstrate which the fluorescent in situ gene protein assay methodology is efficient at resolving cancer gene and protein patterns simultaneously with a cell-by-cell basis, which can be particularly crucial in heterogeneous diseases such cancers.”
The implications on the assay range from the following:
It is particular highly relevant to cancer research due to role of epigenetic and posttranscriptional regulation.
The opportunity to correlate gene and protein information from the same cells might raise the reliability of bookmarker screens.
It will help with therapeutic decision making when screening for less than the gene or perhaps the protein yields indeterminate results.
It can be put on gene-transfection studies which use ‘knock-in’ models to review the effects of gene number on protein expression.